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Update to species: Pao fangi breeding project


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Posted (edited)

Initially identified Pao abei by exporter and eventually found to match Pao fangi and not Pao turgidus as it looked to be  in the aqualog puffer references. There are numerous field guides and studies over the years that have identified Pao puffers as multiple species however after reviewing everything found over the past months I have now identified these as the Pao fangi with high confidence. 

I am far enough along now that I have enough pictures to start this thread. Looking forward to learning as they develop. 

Adult male non-breeding pattern

Screenshot_20231112_110137_VideoPlayer.jpg.d6b72499c6fa61f14603e77a75031d77.jpg

Adult male breeding pattern

Screenshot_20231129_155002_VideoPlayer.jpg.022f29d7f1bc4809a503a4f6fb265b8c.jpg

Screenshot_20240429_210411_VideoPlayer.jpg.0f83ee435d3b902094135818329675c7.jpg

Screenshot_20240429_210922_Instagram.jpg.18cc58931101cbcefe350309217cf8cd.jpg

Adult female breeding coloration

Females seem to have a longer distance from eyes to mouth than males

Screenshot_20240429_210213_VideoPlayer.jpg.b757d829821676a7fcce9f6975ba74aa.jpg

 

29 April 2024 - eggs laid today

female coloration and behavior outside spawning cave. male is seen inside cave guarding spawn (left edge of photo) 

Screenshot_20240429_203837_VideoPlayer.jpg.ea367f9195f57c612c3796b71ebbe4a4.jpg

+/- 20 eggs harvested from cave to coop container with methelyne blue and slow air bubbling through an easy flow  sponge filter collar. based off Pao palustris egg collection I left a portion with the male so he had something to defend.

Egg Container floated in 20 gallon tank for temperature stability. It will be replaced by a german breeding ring once the fry are all swimming

29 April 2024 (Day 0 egg development)

Screenshot_20240429_204000_VideoPlayer.jpg.247fe7e5712662f85df5e93b0988a371.jpg

30 April 2024 (Day 1 - egg development)20240430_Day1PaoTurgidus_1.gif.9e95632f1d314509f52c646b5c9443db.gif

1 May 2024 ( Day 2 - egg development)

Screenshot_20240505_201540_Gallery.jpg.02f8bc45d0c8450b5b50df6523426780.jpg

2 May 2024 ( Day 3 - egg development)

20240502_Day3_1.gif.725f10a111be861737bd863e790f21c0.gif

3 May 2024 (Day 4 - egg development)

20240503_075155_2.gif.4b9aa8484e6e023b42c7afbf3ff861f8.gif

4 May 2024 ( Day 5 - egg development)

20240504_174127_1.gif.71f0164492abcd24f855685c6bd058f6.gif

5 May 2024 (Day 6 - egg development)

Screenshot_20240505_181031_Gallery.jpg.0db746de8c302cee4e52b66f43812e98.jpg

First fry hatched on day 6. Seen below "jumping out of egg,"

20240505_191414_1.gif.67eaf2b0425922285b9d03be8cd5e696.gif

More updates to follow as they develop....

Edited by mountaintoppufferkeeper
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On 5/5/2024 at 10:17 PM, mountaintoppufferkeeper said:

Initially identifed Pao abei by exporter and subsequently found to match Pao turgidus in the aqualog puffer references.

I am far enough along now that I have enough pictures to start this thread. Looking forward to learning as they develop. 

Adult male non-breeding pattern

Screenshot_20231112_110137_VideoPlayer.jpg.d6b72499c6fa61f14603e77a75031d77.jpg

Adult male breeding pattern

Screenshot_20231129_155002_VideoPlayer.jpg.022f29d7f1bc4809a503a4f6fb265b8c.jpg

Screenshot_20240429_210411_VideoPlayer.jpg.0f83ee435d3b902094135818329675c7.jpg

Screenshot_20240429_210922_Instagram.jpg.18cc58931101cbcefe350309217cf8cd.jpg

Adult female breeding coloration

Females seem to have a longer distance from eyes to mouth than males

Screenshot_20240429_210213_VideoPlayer.jpg.b757d829821676a7fcce9f6975ba74aa.jpg

 

29 April 2024 - eggs laid today

female coloration and behavior outside spawning cave. male is seen inside cave guarding spawn (left edge of photo) 

Screenshot_20240429_203837_VideoPlayer.jpg.ea367f9195f57c612c3796b71ebbe4a4.jpg

+/- 20 eggs harvested from cave to coop container with methelyne blue and slow air bubbling through an easy flow  sponge filter collar. based off Pao palustris egg collection I left a portion with the male so he had something to defend.

Egg Container floated in 20 gallon tank for temperature stability. It will be replaced by a german breeding ring once the fry are all swimming

29 April 2024 (Day 0 egg development)

Screenshot_20240429_204000_VideoPlayer.jpg.247fe7e5712662f85df5e93b0988a371.jpg

30 April 2024 (Day 1 - egg development)20240430_Day1PaoTurgidus_1.gif.9e95632f1d314509f52c646b5c9443db.gif

1 May 2024 ( Day 2 - egg development)

Screenshot_20240505_201540_Gallery.jpg.02f8bc45d0c8450b5b50df6523426780.jpg

2 May 2024 ( Day 3 - egg development)

20240502_Day3_1.gif.725f10a111be861737bd863e790f21c0.gif

3 May 2024 (Day 4 - egg development)

20240503_075155_2.gif.4b9aa8484e6e023b42c7afbf3ff861f8.gif

4 May 2024 ( Day 5 - egg development)

20240504_174127_1.gif.71f0164492abcd24f855685c6bd058f6.gif

5 May 2024 (Day 6 - egg development)

Screenshot_20240505_181031_Gallery.jpg.0db746de8c302cee4e52b66f43812e98.jpg

First fry hatched on day 6. Seen below "jumping out of egg,"

20240505_191414_1.gif.67eaf2b0425922285b9d03be8cd5e696.gif

More updates to follow as they develop....

Awesome @mountaintoppufferkeeper

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6 May 2024 (Day 7 most have hatched) ( Day 1 Fry) 

The first to hatch were already starting to hunt today so vinegar eels were added to the container. 1 fry did not survive the night and was removed.

I pulled the collar off an easyflow sponge filter and pushed poly filter in the center to force flow over the resin absorber. This was to try and start pulling excess methelyne blue or organics out of the water and keep the parameters in the container a bit more stable while hatching finishef 

20240506_194842_1.gif.9c4692988faf9b62b96042cfffed53e0.gif

 

7 May 2024 (Day 2)

moved to a short german breeding ring with some vinegar eels and infusoria / copepods for food options. Most fry starting to swim today

20240507_160731_1.gif.e7ef752225679704999353ce31920141.gif

20240507_161032_1.gif.29cbb7b7343ba952d9a05a16d9f3979f.gif

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Posted (edited)

8 May 2024 (Day 3)

A portion of this batch are eating bbs, or grindal worms, or vinegar eels, or copepods. All four options are being fed twice a day

20240508_184117_1.gif.cbea233e06a78846f2c518416244fa38.gif

9 May 2024 (Day 4)

20240509_044935_1.gif.dbc8d0fff592f1b5cb3299087702c8b9.gif

10 May 2024 (Day 5) 

The first baby brine shrimp bellies 

20240510_174644_1.gif.b366b21025fb79af3be5e4947624e3e2.gif

Edited by mountaintoppufferkeeper
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12 May 2024 (Day 7)

1 fry lost who would not eat. I will be adding in MW BW and WW options to future batches and attempt to limit that issue. Of +/- 25 eggs pulled +/- 3 did not hatch 2 did not make it past day 6. I still havent counted the total fry but nearly all of the remainder look great,  look to be  feeding well, and growing quickly. 1 or 2 are much smaller than the rest and they might not develop to adults

Screenshot_20240513_033948_VideoPlayer.jpg.d60c84604857e30074104a55dbae1750.jpg

20240512_193500_1.gif.0cd790e372d36154f93c922acaf56997.gif

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Posted (edited)
On 5/25/2024 at 9:38 AM, Chris said:

Whats the tank maintenance schedule on this 20 gallon? Are you changing much water?

I personally base it off of behaviors of fish and visual cues. I do try and avoid water changes on fry until at least 1/4" long if at all possible.

For the most part I use lots of live plants and light stocking levels help with limiting water changes and the parameter changes they csn bring for me.

I use polyfilter ( id guess any color changing resin based similar media would also work) to provide insurance and a visual guage of water quality in fry tanks. I can watch the speed of the color chage to indicate how much excess nutrients are present. I run duckweed, water lettuce, brazilian pennywort, and subwassertang to help eat the excess nutrients up and maintain stability.

20240525_095508_1.gif.5d7b902c7c3cf5f3079a24057e5b6833.gif

This polyfilter has been in the tank for 48 hours with flow going past it. In my application for fry tanks it will go from white to light brown to dark brown as it absorbs excess organcis. It absorbs other concerns in other applica5ions and changes to different colors depending on what it is absorbing out of the water. 

I do swap out the filter floss in my boxfilters as needed that is normally every 4 or 5 days

I also use ACCR powder as a conditioner normally and have used that many times to block any ammonia spikes due to meds etc 

https://www.aquariumcoop.com/collections/water-care/products/fritz-a-c-c-r-water-conditioner

 

Edited by mountaintoppufferkeeper
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26 May 2024 (Day 20)

The "UFOs" flying around like the ones bottom center to the top right and bottom center to bottom left are scuds. They will be less plentiful when the puffers get to 1/2" TL or so

20240526_190547_2.gif.af6d4ca0166b0c409fe8a81e00bb3058.gif

20240526_190547_1.gif.05e2a33b97022bb87c3046570a7ec270.gif

27 May 2024 (Day 21)

15 fry counted today. They tend to hunt and relax in the subwassertang until the cloud of brine shrimp and grindal worms appears twice a day

20240527_065159_1.gif.30e41741a636dddc1e84dbc1d8db7a21.gif

 

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Posted (edited)

28 May 2024 (Day 22)

The markings on the fry nose and chin on the fry facing the camera were not present in Pao palustris fry; these markings may be unique to the fry of this species. 

20240528_180222_1.gif.a71903f1211dfad2a667179ce41464f1.gif

29 May 2024 (Day 23)

20240529_193410_1.gif.c437d4c57fca5617b5bc4802301a1f0e.gif

30 May 2024 (Day 24)

20240530_191539_1.gif.191b8102515ad7b8163fe278524344eb.gif

Edited by mountaintoppufferkeeper
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I am now not certain on the species I have spawned but will be shortly. The asian puffers can be tough to identify. I have found a few more field collection guides for Laos, Thailand, Cambodia, and one for the Indochinese Mekong which all have some references and photos of Pao puffer species in the region. Based off of that info that i uncovered this morning, these are Pao fangi.

Edited by mountaintoppufferkeeper
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