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Dan
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I have a 75g community tank that’s been up for about 8 months. I want to set up a new 20g tank to separate out the female guppy’s. If I put a new sponge filter in my 75g how long should I wait to use it in the new tank and have it seeded. 

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I've heard from a week to a month.  I split the difference at 2 weeks.  I did push it once at about a week and a half and didnt have an issue with guppies as well.  Also if you can, slowly stock up (dont throw them all in at once).

Then again, your doing guppies, they are a lot more tolerant...

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