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Egg Development Journal


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I was able to pick out a couple medaka eggs and this time they're in methylene blue so hopefully they won't cloud up and die.  I didn't take a photo at 1 day post fertilization but this one is at about the 2 dpf point.

At 1dpf, I could see the oil droplets coalesced into 2 drops (no photo). 

Today (day 2 pf) there's one obvious oil drop, a large yolk and embryo.

PXL_20210818_184026972.jpg.8648492248d4cc9c0bb063c1222d1b95.jpg

 

For reference, below is roughly 1 day(a) and 2 days(b) from an article taken with a much better microscope. 

image.png.ae69d592302c58f79bdeae17596e49d9.png

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Posted (edited)

I was going to do one a day but I can't help my self.  here's T=2.5dpf.  We have eyes now.  The eyes should get black pigment soon (withing hours if what I read was right).  Also the egg has been rolling around collecting more dirt.  They're covered in cilia to help them stick but they also collect dirt.PXL_20210819_063157956(1).jpg.9ef158b7cb9092b6abc17038dc1b73d8.jpg

 

Edited by CT_
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Posted (edited)

Weird, I thought I posted this morning but it didn't go through. Sorry if this gets posted twice.

Here's T=3 dpf.

Today I caught it on its lateral (side) view.  There's a little eye pigmentation now but they should look fully black soon (top photo).  There's also a heartbeat now too!  bottom photo (animated) between the eye and the oil spot.

PXL_20210819_182410090.jpg.88c7a6562f411b51b70d7285ca75012b.jpg

 

PXL_20210819_182736260._exported_stabilized_1629398205422.gif.afeee7e1c3ae9da18ab68b42501f95d7.gif

 

EDIT: adding T=3.5.  eye pigments!

1832668249_PXL_20210820_071458974.2_exported_1629451413135.jpg.ad6d72c9404c428d134a6592f72b54a1.jpg

Edited by CT_
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Posted (edited)

T=6dpf, I think this is a ventral view with the tail wrapped almost all the way around obscuring the eye.  If I mess with the light too much the embryo will twitch and reposition inside the egg. 

I used two sets of tweezers to remove some of the fuzz of what ever is collecting on the egg and was able to get some better photos.

You can now see some of the vasculature around the yolk.  below is a drawing (taken from doi:10.1016/j.mod.2004.03.012) for reference so you know what to look for.

 

 

image.png.51f1f723ef541f78d236b34debd45c2d.png

PXL_20210822_213830072.jpg

Edited by CT_
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T=6.5dpf

I cleaned my microscope a bit, changed the water and detached this guy's sibling from dieing duckweed.  the sibling is a lot cleaner so I'm switching photoing it.

Turns out shallower water and a not terribly dirty microscope goes a long way for photo quality.

 

I took another video too (full video herehttps://streamable.com/v2g21s, quick gif below ).  you can see them twitch some too now and if you look really close you can see blood cells flow (the video compression unfortunately makes that a bit hard).

PXL_20210823_055456510.jpg.0baeda64cbbd56bd7dcd6a8d2e037d99.jpg

 

ezgif.com-gif-maker.gif.958d25cb6cbf725ca12ba578e56b8a42.gif

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wellp.  I took those photos above at about 4pm today and then posted them after dinner.  I looked over about an hour ago(8pm) and all I saw was this:

image.png.25d803a3912a8800cdf462d8c8a3a839.png

Then I looked closer and saw this!

PXL_20210825_035644128.jpg.6850c68a315eb95309d4a2a184cea0a7.jpg

I guess either she had laid and held the eggs an extra day or its hotter than the 27-28C average I thought it was in here.

 

I guess I can test if they'll eat within hours of hatching now.  I've read conflicting things.

 

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Coming up on 48 hr.  Its really hard to see if they're eating but they're doing eating like twitching of their heads (but I don't see anything in their stomach).  The water is barely blue now so I'm going to add some moss after my next water change tonight and some green water in addition to the serra micron just so they have choices.  I also heard they develop better in sunlight (maybe a superstition though) so they're getting 12ish hrs of 10,000 lux (similar to a cloudy day, but feels super bright indoors) light.

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